| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144452 |
| Chromosome: | chromosome 16 |
| Location: | 7716363 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691327 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACGGTAGTCTGCACACTGCTTGTTCCC |
| Internal bar code: | AGGGTTTTACTCTAGATGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 95.0969 |
| LEAP-Seq n confirming: | 2502 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGTATGTCCAGTGATGG |
| Suggested primer 2: | ATATTTGCACACTTTCGCCC |