| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144460 |
| Chromosome: | chromosome 16 |
| Location: | 2034669 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGACCTTCAGGCGCTACTGCGGGTAAG |
| Internal bar code: | GCGAACAATGTACCAGCCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 93.5563 |
| LEAP-Seq n confirming: | 9147 |
| LEAP-Seq n nonconfirming: | 630 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCGTTGCTTTCACCTTA |
| Suggested primer 2: | AGACCCACCTTGTCATCCAG |