| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144469 |
| Chromosome: | chromosome 4 |
| Location: | 1510509 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213800 | (1 of 1) PTHR10825//PTHR10825:SF5 - RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT // E3 UBIQUITIN-PROTEIN LIGASE RING1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACAGCAACACACCTAGCGTTCCAGCTC |
| Internal bar code: | CGAGCCTCATGTCGTTTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 99.6389 |
| LEAP-Seq n confirming: | 2759 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTTGTGTTGCTGCACT |
| Suggested primer 2: | AGCACACAACAGTGTGCCTC |