| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144510 |
| Chromosome: | chromosome 5 |
| Location: | 794217 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248250 | (1 of 1) 1.4.1.24 - 3-dehydroquinate synthase II / DHQ synthase II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGATAGCCCGCCAAGACCTGCTGCCG |
| Internal bar code: | GGGGCACTAGCAGCATTATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 88.5282 |
| LEAP-Seq n confirming: | 18544 |
| LEAP-Seq n nonconfirming: | 2403 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGACCGTGTGTTAGGAA |
| Suggested primer 2: | AGCGTTTATGTTCCGACACC |