| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144518 |
| Chromosome: | chromosome 10 |
| Location: | 2130451 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433476 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTACCTTCCCGGCCTTTCTGATTTCGT |
| Internal bar code: | GGCCTATTTCGAGGAGGTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 99.5099 |
| LEAP-Seq n confirming: | 13806 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGCTAGCATCTCACAA |
| Suggested primer 2: | TGCTGGCTAACGAACACTTG |