| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144536 |
| Chromosome: | chromosome_6 |
| Location: | 4068305 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278212 | CGL46 | Predicted protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTGAGAATGGATCCACTGTTCTCCGGAT |
| Internal bar code: | TCTATGAGTTCGTCCCCGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.6562 |
| LEAP-Seq n confirming: | 2319 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCTAAGCTTTCAATCGT |
| Suggested primer 2: | TGTTCGAGTTCGTGCACTTC |