| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144568 |
| Chromosome: | chromosome 12 |
| Location: | 9275459 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541250 | NAR1E | Putative nitrite transporter; (1 of 7) PTHR30520//PTHR30520:SF0 - FORMATE TRANSPORTER-RELATED // FORMATE TRANSPORTER 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAACCCCACACGCCGCCACGCCCGCACC |
| Internal bar code: | ACCAATGCATTGCGCAAGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 99.6732 |
| LEAP-Seq n confirming: | 5490 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCGGTCGGAATGAGGAG |
| Suggested primer 2: | ACATGGCGACCGTTTCTTAC |