| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144591 |
| Chromosome: | chromosome 16 |
| Location: | 5659178 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679300 | CGL139 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATCACTGCCCGCGGCACTTGCCAGCCTT |
| Internal bar code: | ACAAGCTCGGTTGGAACCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1143 |
| LEAP-Seq percent confirming: | 99.6764 |
| LEAP-Seq n confirming: | 4004 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATCAAAATCTCAGGCCC |
| Suggested primer 2: | AATGCAGGCTCTTGTTGCTT |