| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144597 |
| Chromosome: | chromosome 1 |
| Location: | 526803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002861 | SRR24 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAACGGACTGAGAAGGCGCCCGGCCCCA |
| Internal bar code: | CTGGCAACGGCGTCTCGTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.4606 |
| LEAP-Seq n confirming: | 5900 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCATGTAAATAGGCGGC |
| Suggested primer 2: | CCGCTGGTTTTGGGTTAGTA |