Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.144616 |
Chromosome: | chromosome_10 |
Location: | 6076302 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g463370 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTATGACGCACTGCTGCTGTCTGACAGCG |
Internal bar code: | GGCCTGCACAGCCCTAATGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1098 |
LEAP-Seq percent confirming: | 99.6159 |
LEAP-Seq n confirming: | 14263 |
LEAP-Seq n nonconfirming: | 55 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAACGATGGAACATGGAGA |
Suggested primer 2: | GGTGTGTAGCACCCCTCAGT |