Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.144616 |
Chromosome: | chromosome_10 |
Location: | 6076323 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g463370 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TATCGACGCCCAGCCCAAATAGTCGCGGGT |
Internal bar code: | TGTGGTTAAGCTCCGCATCTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 682 |
LEAP-Seq percent confirming: | 99.9256 |
LEAP-Seq n confirming: | 10751 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAACGATGGAACATGGAGA |
Suggested primer 2: | GGTGTGTAGCACCCCTCAGT |