Insertion junction: LMJ.RY0402.144621_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.144621
Chromosome:chromosome 3
Location:3622031
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g168650 (1 of 1) PF08621 - RPAP1-like, N-terminal (RPAP1_N) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACATTTCAGCCACGCATCCGCGCGACCGC
Internal bar code:AGGGAAGGTAGGGGGCGTGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:579
LEAP-Seq percent confirming:81.6811
LEAP-Seq n confirming:5199
LEAP-Seq n nonconfirming:1166
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGAAGCTTTGGTCTCAGG
Suggested primer 2:GACTCAGACAAGCAGGAGGG