| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144629 |
| Chromosome: | chromosome 10 |
| Location: | 790303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423200 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACGAGGACGCGGCGGATGAGGATGCG |
| Internal bar code: | TATGACGCAAGAGTGCGCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 156 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTACCTGAGACGAAGC |
| Suggested primer 2: | TTATCAGAGCCCACTCCGAC |