| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144629 |
| Chromosome: | chromosome 14 |
| Location: | 2938329 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627650 | FAP392 | Flagellar Associated Protein 392 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAGCCTTCCACTTGACGCGCACCAAAC |
| Internal bar code: | TGCACGAGGGCGGGCCACATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCCAGAGGCGGAACATC |
| Suggested primer 2: | ATCAACAGTCCACAGGGAGG |