| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144637 |
| Chromosome: | chromosome 9 |
| Location: | 5247978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399626 | (1 of 1) K09919 - hypothetical protein (K09919) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTGTCCAGGGAGCGCGGGCAGATCGAA |
| Internal bar code: | GACTCTTGATGTTCGGGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCAACCTGCTGTCACTC |
| Suggested primer 2: | AGTTGAATGCTCAGGTCGCT |