| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144637 |
| Chromosome: | chromosome 12 |
| Location: | 1864654 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511600 | (1 of 1) PTHR13196//PTHR13196:SF14 - DENN DOMAIN-CONTAINING // PROTEIN RME-4, ISOFORM A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACACCGCACTGCAGGTCCTCCAAGTCGC |
| Internal bar code: | GTGGCTACGCAACCCAGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.7472 |
| LEAP-Seq n confirming: | 789 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCCCTCATCACTACGGC |
| Suggested primer 2: | TTAAGCGAGCTCGAGAGGAG |