| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144641 |
| Chromosome: | chromosome 1 |
| Location: | 1472689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007550 | GT90-1,GT90F1 | GT90 family protein 1; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAAACCACATCACTGTCGCCACTTCAC |
| Internal bar code: | TAGCGCATGAAAGAAAACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.2445 |
| LEAP-Seq n confirming: | 11428 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGCAAGAAAAGGAGAAC |
| Suggested primer 2: | CAAACTCCCTGCAAAAGAGC |