| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144658 |
| Chromosome: | chromosome 10 |
| Location: | 2972835 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440550 | (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTGGCTCGTATCATACATTGCTGCGGG |
| Internal bar code: | GTCAGCGGTGTCGACGTGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 93.6611 |
| LEAP-Seq n confirming: | 3753 |
| LEAP-Seq n nonconfirming: | 254 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTCCCGCTTACAGGTTT |
| Suggested primer 2: | TGTTCATCGCAGCGTTCTTA |