| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144661 |
| Chromosome: | chromosome 2 |
| Location: | 2326657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090700 | MAPKKK8 | Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCAGCAAGGCCAGTCACGACTGTCGCC |
| Internal bar code: | GATGGCAATCGTACCGCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 87.4673 |
| LEAP-Seq n confirming: | 8696 |
| LEAP-Seq n nonconfirming: | 1246 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCACACACCCTGTATCAT |
| Suggested primer 2: | GCTGTCAAAGCACGTACGAA |