| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144669 |
| Chromosome: | chromosome 10 |
| Location: | 4887371 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454550 | COX19 | (1 of 1) K18183 - cytochrome c oxidase assembly protein subunit 19 (COX19); Mitochondrial cytochrome c oxidase assembly protein | 3'UTR|3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCTAAGGGTTAGGTCCGCGCGGCTATA |
| Internal bar code: | CACCACCGTAACATCGCATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 99.6631 |
| LEAP-Seq n confirming: | 6509 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTCAAACAGCAAATAGCA |
| Suggested primer 2: | CGCGCTGTATAGACCTGTCA |