| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144689 |
| Chromosome: | chromosome 6 |
| Location: | 7150025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297550 | (1 of 1) K14649 - transcription initiation factor TFIID subunit 8 (TAF8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCGCACCTGCTCGGCAATCCAGCTGT |
| Internal bar code: | AATAGTGTGGAAGGGTCCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 94.3662 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGCATTAGCGGAAGTG |
| Suggested primer 2: | ACTGGCCTATTTGACGGTTG |