| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144694 |
| Chromosome: | chromosome_6 |
| Location: | 974406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g255700 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGACATGGAGCAGAACGGCCCCGTGCCCGC |
| Internal bar code: | GGTCCACTTTCGGGCCAATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 91.3078 |
| LEAP-Seq n confirming: | 1145 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGACTTGCACCTCTTCTG |
| Suggested primer 2: | TGCCGTTTACACTGGATTGA |