Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.144694 |
Chromosome: | chromosome_9 |
Location: | 4488955 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g395769 | FAP177 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGCTGCACAGTACGCACCTTGATTGCCAG |
Internal bar code: | GTATTTCGCGCCATCTTGTGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 453 |
LEAP-Seq percent confirming: | 94.6429 |
LEAP-Seq n confirming: | 848 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCAGTGCTATCAAACCGCA |
Suggested primer 2: | CCCTCTCCTTTCCTTCCTTG |