| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144694 |
| Chromosome: | chromosome 9 |
| Location: | 4488955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395769 | FAP177 | Flagellar Associated Protein 177 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTGCACAGTACGCACCTTGATTGCCAG |
| Internal bar code: | GTATTTCGCGCCATCTTGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGTGCTATCAAACCGCA |
| Suggested primer 2: | CCCTCTCCTTTCCTTCCTTG |