| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144701 |
| Chromosome: | chromosome 12 |
| Location: | 6421484 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537750 | (1 of 1) PTHR22957:SF34 - TBC1 DOMAIN FAMILY MEMBER 25 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTTGCCGTACCCCGCACGTTCTGTAC |
| Internal bar code: | GAATGACGAACGTCGAGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 30 |
| LEAP-Seq percent confirming: | 83.0601 |
| LEAP-Seq n confirming: | 152 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACACCGATCTCTCACA |
| Suggested primer 2: | GAAACGAATTAAGCAGGGCA |