| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144704 |
| Chromosome: | chromosome_1 |
| Location: | 3647927 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g023550 | FAP234 | Flagellar Associated Protein, putative outer arm dynein light ch | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTCGTCGCCCAACCGGGGGAGCTGGGGTG |
| Internal bar code: | ACGTTAAATTCTCTCGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 78.5918 |
| LEAP-Seq n confirming: | 3304 |
| LEAP-Seq n nonconfirming: | 900 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCAAAGGGACTGCTTGAC |
| Suggested primer 2: | GGTAATCCCAGCTGAACCAA |