| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144721 |
| Chromosome: | chromosome 3 |
| Location: | 842010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146507 | SPT2 | (1 of 2) 2.3.1.50 - Serine C-palmitoyltransferase; Serine palmitoyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAATGGGCCCTGGGACCCTTTGTGTGC |
| Internal bar code: | GGGTCACCGCCAGGACAGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.52 |
| LEAP-Seq n confirming: | 4354 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAACTTCGACCGTCATT |
| Suggested primer 2: | CACATGACAATAGCGGGTTG |