| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144729 |
| Chromosome: | chromosome 12 |
| Location: | 6261021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537050 | NUOA3 | NADH:ubiquinone oxidoreductase subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGACACGTGTTTCAGGTGGGACAGCAG |
| Internal bar code: | GTACGAGTCTCCACCGGTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 99.7685 |
| LEAP-Seq n confirming: | 1293 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGTGCTGTAGGAAAGGC |
| Suggested primer 2: | CGTCGGTAAGGAGCTTTCTG |