| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144733 |
| Chromosome: | chromosome 3 |
| Location: | 8791313 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205809 | (1 of 1) PF01367//PF02739 - 5'-3' exonuclease, C-terminal SAM fold (5_3_exonuc) // 5'-3' exonuclease, N-terminal resolvase-like domain (5_3_exonuc_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTCCGAGTCGTCGCCCGTCAGTGCCTT |
| Internal bar code: | TAGAGCCGCTCCCTTGAACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGACTTCCGGAACCTG |
| Suggested primer 2: | TACGGAAGTGGCAGAGAGGT |