| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144752 |
| Chromosome: | chromosome 1 |
| Location: | 5303655 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037100 | (1 of 4) PTHR24347//PTHR24347:SF138 - SERINE/THREONINE-PROTEIN KINASE // PROTEIN F32D8.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCAGGTAATGAGTGCGGTTGCAAAGCC |
| Internal bar code: | TACTTTTTGGGCCTTTGTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.4867 |
| LEAP-Seq n confirming: | 14343 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACTGAAGCTCGACTGAC |
| Suggested primer 2: | CATAAGCTGCACGTCCAAGA |