| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144771 |
| Chromosome: | chromosome 3 |
| Location: | 386121 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144887 | (1 of 1) IPR000048//IPR003590//IPR006553 - IQ motif, EF-hand binding site // Leucine-rich repeat, ribonuclease inhibitor subtype // Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGTGGGTTTGGGTCAGCGCCACTCAGG |
| Internal bar code: | GCCAGTGCACCGGGTTGCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1205 |
| LEAP-Seq percent confirming: | 99.2273 |
| LEAP-Seq n confirming: | 18750 |
| LEAP-Seq n nonconfirming: | 146 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTTAAGCATGCTGGTC |
| Suggested primer 2: | GTACACACGCAATGGTTTCG |