| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144798 |
| Chromosome: | chromosome 16 |
| Location: | 6689040 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671937 | (1 of 1) PF05990 - Alpha/beta hydrolase of unknown function (DUF900) (DUF900) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGTGCACCGGGTCGCCAGAAAGTGCT |
| Internal bar code: | AAGCTGCACCAGGCTCGGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 56.1739 |
| LEAP-Seq n confirming: | 1938 |
| LEAP-Seq n nonconfirming: | 1512 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGCAGGAGCGACACTA |
| Suggested primer 2: | CGTAGGAGGAGCTGAAGGTG |