| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144800 |
| Chromosome: | chromosome 11 |
| Location: | 2555265 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476100 | OPR50,MCD1 | petD mRNA stabilization factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATTTCGCGGGCCGGGTAGCCGAAGCGG |
| Internal bar code: | GGGGGGACGGGGGTAGGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.8166 |
| LEAP-Seq n confirming: | 4898 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAGGACCCACAACTGCT |
| Suggested primer 2: | CGACTTTTCCAAGGATCCAA |