| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144805 |
| Chromosome: | chromosome_2 |
| Location: | 3519905 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g095126 | DPE2 | alpha-1,4-glucanotransferase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCGGAAGTCCGGGACACCTGTATGGCCG |
| Internal bar code: | TGTAGAATTGTAGTAAGGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 965 |
| LEAP-Seq percent confirming: | 99.6901 |
| LEAP-Seq n confirming: | 3538 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGGGTTAAGCTGTTGTC |
| Suggested primer 2: | TCCAAACCTGAAGCATTTCC |