| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144820 |
| Chromosome: | chromosome 3 |
| Location: | 1735667 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAACAGTCATCACACAACGTCACCTCC |
| Internal bar code: | GCTTCTGTTGCGGTATTTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1077 |
| LEAP-Seq percent confirming: | 99.8085 |
| LEAP-Seq n confirming: | 3648 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACATGAGGTTTCTGAGCG |
| Suggested primer 2: | GCTACCTGCTCCTCACGTTC |