| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144823 |
| Chromosome: | chromosome_17 |
| Location: | 5508830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGATTCTCTTCCGCTAAGCTTGCTACGCC |
| Internal bar code: | GACCATTCAAAACGCAACGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.2825 |
| LEAP-Seq n confirming: | 3321 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACATACTGTGCAGTGCT |
| Suggested primer 2: | GACCTGCTTGCTTTCCTGTC |