Insertion junction: LMJ.RY0402.144843_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.144843
Chromosome:chromosome 7
Location:2428712
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328900 CDPK3,CDPK9 (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTCCACATGTCCGCCTCGTGGCTGCGCG
Internal bar code:TTCGACTAAAGGGCGGTGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGAGGGATAGCGGACATC
Suggested primer 2:TCCGTCTTGAAGTCGATGTG