| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144849 |
| Chromosome: | chromosome 2 |
| Location: | 6707137 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGTAGGGTGACAGCAACCTGGCCAGG |
| Internal bar code: | GGGTGTCCGTCCCGTCGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 99.844 |
| LEAP-Seq n confirming: | 8959 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGTTCCTAGGCTGCTTG |
| Suggested primer 2: | AAGGTTGATGCAAACCTTGG |