Insertion junction: LMJ.RY0402.144867_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144867
Chromosome:chromosome 17
Location:4128380
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCATTCCCGAAGTCGCCCACTAGCAGTG
Internal bar code:CGCGTGGACGCAGCGCGAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:99.669
LEAP-Seq n confirming:2710
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACCCCTGCAGCTACTGA
Suggested primer 2:TCAATCGATGCTTTGTAGCG