| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144881 |
| Chromosome: | chromosome 3 |
| Location: | 3459919 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167400 | (1 of 1) PF11282 - Protein of unknown function (DUF3082) (DUF3082) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCGAGTTATTGCATCGCCGTCAGCGC |
| Internal bar code: | GCCATCTAAGTTCCGGAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 99.0674 |
| LEAP-Seq n confirming: | 1381 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCAACTGCTGTTCTTCC |
| Suggested primer 2: | CGGATGAGCTGTGTAGACGA |