Insertion junction: LMJ.RY0402.144883_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.144883
Chromosome:chromosome 1
Location:5555902
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g039500 FAP89 Flagellar Associated Protein 89; (1 of 3) PTHR22847:SF361 - JOUBERIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAAATTGCGGTCCTCGCTTTCATCCCAC
Internal bar code:GACTCGCGGAGTGCGCCCGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:887
LEAP-Seq percent confirming:99.6761
LEAP-Seq n confirming:9232
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATTGCTTTACAGGTGGT
Suggested primer 2:GCCTGATTGGTACCGTGACT