| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144887 |
| Chromosome: | chromosome_17 |
| Location: | 3367422 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g723600 | IFT81 | Intraflagellar transport protein 81 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGAGTCCCGCTTGCCCGCGACTGTTTAG |
| Internal bar code: | ATGAGCGGTGAGGTGCAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 99.9236 |
| LEAP-Seq n confirming: | 5233 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACTCGTCAATAACGGGC |
| Suggested primer 2: | ATCGGGGAAGCTCAGGTAGT |