| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144910 |
| Chromosome: | chromosome 4 |
| Location: | 3400168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227526 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGAAGCCAGCCTAAGCCATGCATGTA |
| Internal bar code: | GGAGGTGGCGTCACGACGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.2419 |
| LEAP-Seq n confirming: | 4451 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCGAAGAGCACAACCT |
| Suggested primer 2: | TTGCTGCTTTGGATGTTGAG |