| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144941 |
| Chromosome: | chromosome_14 |
| Location: | 2672627 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g626466 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCCGCCTCCTAGGCGCCCCAGACCCCCCC |
| Internal bar code: | TGGGTGAAGCGTCGGTACTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGGTTAGTTTGGTTCA |
| Suggested primer 2: | GTAGTGGCGGTAGGGCTATG |