| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144950 |
| Chromosome: | chromosome 13 |
| Location: | 3944236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590800 | HTA21,HTA37 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACAAGCCAAGTCCACAAGACCGAGCGC |
| Internal bar code: | CGTAGAAGATGTCCAACTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 99.0025 |
| LEAP-Seq n confirming: | 397 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGAAGGGCAAGTACGC |
| Suggested primer 2: | CTAGAGAGGGACAGCATCGG |