Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.144968 |
Chromosome: | chromosome_17 |
Location: | 3426842 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g724400 | FAP257 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGGAGTTCGGGCGCTGCGCATAGCGGCTG |
Internal bar code: | CGCGGCGTAGTGAAAGCTAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 943 |
LEAP-Seq percent confirming: | 99.7074 |
LEAP-Seq n confirming: | 1704 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGGATTCAGGAGATTACGA |
Suggested primer 2: | CATTAATTCGAGAGCAGGGC |