| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144968 |
| Chromosome: | chromosome_17 |
| Location: | 3426851 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724400 | FAP257 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGGCATACTCCTCCTAGGGCAAACTCAC |
| Internal bar code: | CGCGCCGTCCCCGGGGCAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 18.0611 |
| LEAP-Seq n confirming: | 1496 |
| LEAP-Seq n nonconfirming: | 6787 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGATTCAGGAGATTACGA |
| Suggested primer 2: | CATTAATTCGAGAGCAGGGC |