| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144981 |
| Chromosome: | chromosome 6 |
| Location: | 3861294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278176 | (1 of 1) PTHR22847//PTHR22847:SF500 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCATCCCCTCTGGCTGCCAGCAATGGG |
| Internal bar code: | CGACGTCCTGTACGAGGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 97.1631 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTCTTCAAAGCAAGACC |
| Suggested primer 2: | CTGCTGGAGCTGTGTGATGT |