| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144986 |
| Chromosome: | chromosome 13 |
| Location: | 262845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563800 | MYO3 | (1 of 3) K10357 - myosin V (MYO5); Myosin heavy chain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTGTTGGGTTCACTGCAGCCCGCAATC |
| Internal bar code: | ATCCGGTCGTGGACTTTAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 98.9007 |
| LEAP-Seq n confirming: | 2519 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATGCCAAACAGGAGTAT |
| Suggested primer 2: | ATTGGAGCCGTGGTTTGTAG |