Insertion junction: LMJ.RY0402.144989_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144989
Chromosome:chromosome 6
Location:2281137
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g266900 LPA1L Low PSII Accumulation 1 homolog; (1 of 2) PF11998 - Protein of unknown function (DUF3493) (DUF3493) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTTGAACTCCACGCTGTGATACACTGGT
Internal bar code:TATCGTGCAACCGTAGGGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:822
LEAP-Seq percent confirming:97.9721
LEAP-Seq n confirming:2174
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTCCTGGTTTGAGCAGC
Suggested primer 2:GAAGGCCTGCTTCACAAGTC