Insertion junction: LMJ.RY0402.145001_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145001
Chromosome:chromosome 17
Location:5307189
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g736300 (1 of 1) IPR000104//IPR011047//IPR018391 - Antifreeze protein, type I // Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACACACCCGCACGCATCCGCAACTCCCA
Internal bar code:GCAGCCAACCCGACCATCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:289
LEAP-Seq percent confirming:99.0142
LEAP-Seq n confirming:8035
LEAP-Seq n nonconfirming:80
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTTCACCTCGATCCTCAC
Suggested primer 2:AACACCACACAAACCCAACA